ABSTRACT

Tracheal segments cryopreservation: Effect of freezing time on the structural integrity of cartilage. Pathological long tracheal segments (› 6 cm) remain an unsolved medical issue, since long lesions are not amenable to perform an end to end anastomosis. Such pathologies include tumors, post-intubation stenosis, malacias, tracheoesophagic fistulae and many others. There are many research groups that are working to solve this challenge, focused on tracheal cryopreservation or tissue-engineered tracheal transplant. In this work, we study the histological changes on the trachea tissue that result from different cryopreservation time, in order to obtain information that could let us perform tracheal transplantation in the near future.

KEYWORDS

Trachea, tracheal transplantation, cryopreservation.

REFERENCES

1. Villalba CJ. Respuesta tisular del aloinjerto traqueal liofilizado y criopreservado combinado con terapia de inmunosupresión y aplicación tópica del factor de crecimiento del endotelio vascular [tesis doctoral]. México, D.F.: Escuela Superior de Medicina, IPN; 2007.

2. Sotres VA. Evaluación de las alteraciones macroscópicas, microscópicas y en la expresión de caspasa-3 en el tejido traqueal criopreservado por efecto de la solución, de la temperatura y del tiempo de congelación [tesis doctoral]. México, D.F.: UAM-X; 2010.

3. Sotres-Vega A, Villalba-Caloca J, Jasso-Victoria R, et ál. Cryopreserved tracheal grafts: a review of the literature. J Invest Surg 2006;19:129-135.

4. Guyton CA, Hall EJ. Tratado de fisiología médica. 10 ed. México: McGraw-Hill-Interamericana; 2005.p.534-536.

5. Junqueira LC, Carneiro J. Histología básica. Texto y Atlas. Barcelona, España: Masson; 2001; 83-348.

6. Nakanishi R, Umesue M, Hashimoto M, Muranaka H, Hachida M, Yasumoto K. Limit of warm ischemia time before cryopreservation in rat tracheal isografts. Ann Thorac Surg 2000;70:1800-1804.

7. Hashimoto M, Nakanishi R, Umesue M, Muranaka H, Hachida M, Yasumoto K. Feasibility of cryopreserved tracheal xenotransplants with the use of short-course immunosuppression. J Thorac Cardiovasc Surg 2001;121:241-248.

8. Inutsuka K, Kawahara K, Takachi T, Okabayashi K, Shiraishi T, Shirakusa T. Reconstruction of trachea and carina with immediate or cryopreserved allografts in dogs. Ann Thorac Surg 1996;62:1480-1484.

9. Nakanishi R, Hashimoto M, Muranaka H, Umesue M, Kohno H, Yasumoto K. Maximal period of cryopreservation with the Bicell biofreezing vessel for rat tracheal isografts. J Thorac Cardiovasc Surg 1999;117:1071-1076.

10. Kushibe K, Nezu K, Nishizaki K, Takahama M, Taniguchi S. Tracheal allotransplantation maintaining cartilage viability with long-term cryopreserved allografts. Ann Thorac Surg 2001;71:1666-1669.

11. Kushibe K, Tojo T, Sakaguchi H, et ál. Assessment of cartilage viability in the cryopreserved tracheal allograft by measurement of Na(2)(35)SO(4) Incorporation. Transplant Proc 2000;32:1655-1656.

12. Diario Oficial de la Federación. Especificaciones técnicas para la producción cuidado y uso de animales de laboratorio de la Norma Oficial Mexicana NOM-062-ZOO-1999. Diciembre 6. Estados Unidos Mexicanos: Diario Oficial de la Federación; 1999.

13. Guide for Care and Use of Laboratory Animals. U.S. Department of Health and Human Services, Public Health Service, National Institutes of Health; 1985.

14. Murakawa T, Nakajima J, Motomura N, Murakimi A, Takamoto S. Successful allotransplantation of cryopreserved tracheal grafts with preservation of the pars membranacea in nonhuman primates. J Thorac Cardiovasc Surg 2002;123:153-160.

15. Nakanishi R, Onitsuka T, Shigematsu Y, Hashimoto M, Muranaka H, Yasumoto K. The immunomodulatory effect of cryopreservation in rat tracheal allotransplantation. J Heart Lung Transplant 2002;21:890-898.

16. Tanaka H, Maeda K, Okita Y. Transplantation of the cryopreserved tracheal allograft in growing rabbits. J Pediatr Surg 2003;38:1707-1711.

17. Lenot B, Macchiarini P, Dulmet E, Weiss M, Dartevelle P. Tracheal allograft replacement. An unsuccessful method. Eur J Cardiothorac Surg 1993;7:648-652.

18. Mukaida T, Shimizu N, Aoe M, Andou A, Date H. Tracheal allotransplantation after varying terms of cryopreservation. Transplant Proc 1998;30:3397-4000.

19. Zhao H, Hua TC, Zhou YZ, Wang QF, Yang Y, Bao LL. Cryopreservation and transplantation of dog trachea. Ann NY Acad Sci 1998;858:270-275.

20. Kumon BC, Shiraishi T, Makihata S, Kawahara K, Shirakusa T. Cartilaginous injury limits cryopreservation of tracheal isograft. Respirology 2000;5:105-110.