ABSTRACT

Objective: To evaluate a BacT ALERT 3D automatic system for mycobacteria isolates. Methods: Were study 819 clinical samples received at the National Reference Laboratory of Tuberculosis, IPK, from August 2010 to November 2011. The samples were inoculated in parallel on Löwenstein Jensen medium and BacT ALERT 3D automatic system. The results obtained were analyzed and compared by number of isolates, time detection of growth (TDG), contamination rate (CR) and calculated the quality indicators of BacT ALERT 3D automatic system. Results: By Löwenstein Jensen (LJ) was obtained 99 (90%) positives isolates, 102 (92.7%) by BacT ALERT 3D and 89 (80.9%) by two methods simultaneously. El TDG average of BacT ALERT 3D was 16.67 days and 22.50 days for LJ. The CR was 7.4% and 6.7 % for LJ and BacT ALERT 3D, respectively. The concordance between BacT ALERT 3D and LJ was 97.82%. The sensitivity, specificity and Youden index obtained by BacT/ALERT 3D was 97.75%, 98.44% and 0.92, respectively. Conclusions: BacT/ALERT 3D system is a suitable method for recovering mycobacteria from clinical samples. It demonstrated a shorter TDG of mycobacteria of LJ medium. The LJ culture it must be used in combination with automatic systems for to assure a mycobacterial Mycobacterium recovery.

KEYWORDS

Tuberculosis, Bact/ALERT 3D system, Löwenstein Jensen, culture.

REFERENCES

1. Solorzano A, Soria I, Roman J, et al. Comparative evaluation of three culture methods for the isolation of mycobacteria from clinical samples. J Microbiol Biotechnol 2009;19:1259-1264.

2. Parrish N, Dionne K, Sweeney A, Hedgepeth A, Carroll K. Differences in time to detection and recovery of Mycobacterium spp. between the MGIT 960 and BacT/ALERT MB automated culture systems. Diagn Microbiol Infect Dis 2009;63:342-345.

3. Piersimoni C, Scarparo C, Callegaro A, et al. Comparison of MB/BacT ALERT 3D system with radiometric BACTEC system and Löwenstein-Jensen medium for recovery and identification of mycobacteria from clinical specimens: a multicenter study. J Clin Microbiol 2001;39:651-657.

4. Rohner P, Pepey B, Auckenthaler R. Comparison of BacT/Alert with Signal blood culture system. J Clin Microbiol 1995;33:313-317.

5. Drobniewski FA, Caws M, Gibson A, Young D. Modern laboratory diagnosis of tuberculosis. Lancet Infect Dis 2003;3:141-147.

6. Marrero A, Carreras L, Valdivia JA, et al. Programa Nacional de Control de la Tuberculosis. Manual de normas y procedimientos. La Habana, Cuba: Ciencias Médicas, 2010.

7. Organización Panamericana de la Salud. Manual para el Diagnóstico Bacteriológico de la Tuberculosis. Normas y guía técnica. Parte II. Cultivo. OPS, 2008.

8. Gaillard T, Fabre M, Martinaud C, Vong R, Brisou P, Soler C. Assessment of the SD Bioline Ag MPT64 Rapid™ and the MGIT™ TBc identification tests for the diagnosis of tuberculosis. Diagn Microbiol Infect Dis 2011;70:154-156.

9. Werngren J, Klintz L, Hoffner SE. Evaluation of a novel kit for use with the BacT/ALERT 3D system for drug susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol 2006;44:2130-2132.

10. Agudelo CA, Builes LN, Hernández M, Robledo J. Nuevos métodos para el diagnóstico de la tuberculosis. IATREIA 2008;21:321-332.

11. Harris G, Rayner A, Blair J, Watt B. Comparison of three isolation systems for the culture of mycobacteria from respiratory and non-respiratory samples. J Clin Pathol 2000;53:615-618.

12. Recommendations of the Advisory Council for the Elimination of Tuberculosis. Essential components of a tuberculosis prevention and control program. MMWR Recomm Rep 1995;44(RR-11):1-16.

13. Alfa MJ, Manickam K, Sepehri S, Sitter D, Lenton P. Evaluation of BacT/Alert 3D automated unit for detection of nontuberculous mycobacteria requiring incubation at 30 degrees C for optimal growth. J Clin Microbiol 2011;49:2691-2693.

14. Centers for Disease Control and Prevention (CDC). Updated guidelines for the use of nucleic acid amplification tests in the diagnosis of tuberculosis. MMWR Morb Mortal Wkly Rep 2009;58:7-10.

15. Somoskovi A, Mester J, Hale YM, Parsons LM, Salfinger M. Laboratory diagnosis of nontuberculous mycobacteria. Clin Chest Med 2002;23:585-597.

16. Alcaide F, Benítez MA, Escribà JM, Martín R. Evaluation of the BACTEC MGIT 960 and MB/BacT systems for recovery of mycobacteria from clinical specimens and for species identification by DNA AccuProbe. J Clin Microbiol 2000;38:398-401.

17. Dionne K, Sweeney A, Hedgepeth A, Carroll K, Parrish N. Methods for reducing bacterial contamination in the BacT/Alert mycobacterial culture detection system. J Clin Microbiol 2005;43:2523-2525.

18. Carricajo A, Fonsale N, Vautrin AC, Aubert G. Evaluation of BacT/Alert 3D liquid culture system for recovery of mycobacteria from clinical specimens using sodium dodecyl (lauryl) sulfate-NaOH decontamination. J Clin Microbiol 2001;39:3799-3800.

19. Garrigó M, Aragón LM, Alcaide F, et al. Multicenter laboratory evaluation of the MB/BacT Mycobacterium detection system and the BACTEC MGIT 960 system in comparison with the BACTEC 460TB system for susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol 2007;45:1766-1770.