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Effect of moderate level of superimposed alveolar hypoxia on the rate of edema formation and proteins transport in a canine isolated lobe model of hydrostatic pulmonary edema, using spectrophotometric methods

Andrés Palomar, Rosamelia Fernández, Arturo Gómez, Nora G. Krüll, Rafael Harari, Nancy Gertrudiz, María Luisa Martínez Guerra, Julio Sandoval, Luis Oppenheimer





2003, Number 1

2003; 62 (1)






ABSTRACT

Effect of moderate level of superimposed alveolar hypoxia on the rate of edema formation and proteins transport in a canine lobal lobe model of hydrostatic pulmonary edema, using the spectrophotometric method. Several mechanisms have been implicated in the genesis of pulmonary edema related to alveolar hypoxia. In this experimental model, we studied the pulmonary edema formation at different oxygen inspiratory fractions. In 12 isolated blood perfused and statically inflated canine left lower lobes, we measured continuously the rate of transvascular fluid exchange (Qf) at two levels of inspiratory oxygen fraction (FIO2) and the same inspiratory CO2 fraction, Qf was continuously determined from changes in light transmission (815 nm) proportional to changes in hematocrit. We increased Qf by changes in hydrostatic pressure (capillary pressure 25.2 ± 0.74 cm H2O). The Qf measurements were performed during two conditions in all lobes. Normoxia = FIO2 21% and hypoxia = FIO2 5%. Seven lobes started with normoxia and 5 with hypoxia. Both conditions lasted 30 minutes. Results: The PaO2 was significantly different between hypoxia (49.28 ± 7.8 mmHg) and normoxia (123 ± 24.1 mmHg). The Qf during normoxia was 0.469 ±0.33 and 0.358 ± 0.2mL/min/100 g during hypoxia, P = NS. Qf as assessed by continuous weight recording was also not different in both conditions. In a second group of experiments using the same protocol, in eight separate experiments we calculated the reflection coefficients of the membrane (σ), using dyed plasma proteins with Evans blue and calculating the plasma concentration with spectrophotometry at 610 nm. The σ during normoxia was 0.66± 0.17 and 0.63 ± 0.21 in hypoxia P = NS. Conclusion: In this model of pulmonary edema, moderate alveolar hypoxia does not produce any significant change in Qf and σ.

KEYWORDS

Alveolar hypoxia, spectrophotometric method.

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2003, Number 1

2003; 62 (1)