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Effect of the SNP -197G›A in the production of IL-17A in response to hyper-virulent strains of M. tuberculosis

Espinosa-Soto, Ramón; Regino-Zamarripa, Nora Elemi; León-Ávila, Gloria; Giono-Cerezo, Silvia; Muñoz-Torrico, Marcela; Salazar-Lezama, Miguel Ángel; Márquez-García, Eduardo; García-Sancho, María E; Cruz-Lagunas, Alfredo; Mendoza-Milla, Criselda; Jiménez-Álvarez, Luis A; Ramírez-Martínez, Gustavo; Zúñiga, Joaquín





2017, Number 3

2017; 76 (3)






ABSTRACT

Background: Th1/Th2 immunity is insufficient to control Mycobacterium tuberculosis (Mtb) infection, particularly against more virulent strains such as W-Beijing. Methods: Thirty patients with active tuberculosis (TBA) and 30 controls with latent tuberculosis (TBL) were included. The distribution of polymorphism rs2275913 was evaluated by real-time PCR. Mononuclear cells (MNCs) were stimulated with CFP-10 and ESAT-6 antigens and extracts of H37Rv, W-Beijing-HN878. Levels of IL-17, IFN-γ were quantified by Luminex. Differences were assessed with Student t and U of Mann-Whitney and p values ‹ 0.05 were significant. Results: No significant association of the 197G›A polymorphism with susceptibility to TBA was found. An effect of the -197G›A mutation was observed on the IL-17A expression rate. The MNCs of TBA patients with A/G genotype showed higher IL-17A (185 pg/mL) production relative to G/G patients (100 pg/mL, p = 0.008). This was not observed in individuals with TBL. The antigens of H37Rv and HN878 stimulated higher production of IFN-γ in MNCs of patients with ATB (p ‹ 0.05). Conclusion: The presence of the A allele at the -197 position of the IL-17A promoter conditions increased production of this cytokine in response to the W-Beijing-HN878 hypervirulent strain.

KEYWORDS

IL-17, -197G›A polymorphism, tuberculosis, W-Beijing HN878.

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2017, Number 3

2017; 76 (3)